Part:BBa_K1897003

HasS antisigma factor coding sequence

Usage and Biology

HasS (34.7 kDa) is the anti-sigma factor in the Has system signalling cascade. The Has system is a heme acquisition system originally from the Serratia marcescens. HasS is responsible for binding to the cytoplasmic extra cytoplasmic function sigma factor, HasI, inhibiting its activity. When the holo-HasA hemophore binds to the cell surface receptor HasR, it triggers a conformational change in HasS. This then releases the HasI which allows for the expression of genes under the control of the Has promoter.

Characterisation of this part is done in tandem with the full has operon (BBa_K1897007)

References

Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition. Molecular microbiology, 53(4), 1267-1277.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 34
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 317
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 72
  
• 1000
  COMPATIBLE WITH RFC[1000]